LunarPIXImus光密度计LunarCorp.MadisonWIDEXAPIXImus骨密度仪双能X-线吸收仪Academicandpharmaceuticalresearcherscommonlyusethemouseforinvestigationsofgeneticscellularphysiologyandofagentsaffectingboneorsoft-tissuecomposition.Boneandtissuecompositionmeasurementusingdual-energyx-rayabsorptiometry(DEXA)densitometryeliminatetheneedfordestructivechemicalanalysis-atime-andlabor-consumingprocessthatrequiresdaysorweekstocomplete.DEXAdensitometryallowstheresearchertheopportunitytomakemultiplemeasurementsinsituduringthelifeoftheanimal-providingtruelongitudinalmeasures.UnfortunatelythespatialresolutionoftableDEXAdensitometersistoopoorformiceandtherectilinearscanningprocessofolderperipheralDEXAdensitometersrequireasmuchas30minutesforatotalbodyacquisitionwithrelativelypoorprecisionandspatialresolution.Thelongprocessrequirescarefulsedationtechniquesandoftenendangersanimalsafety.TheLunarPIXImusDensitometer(GEMedicalSystems)providesbonemineralandbodycompositionresultsfromtotalbodyimaginginlessthan5minutes.Fastimagingallowsfasteraccesstoimportantdataandissaferonanimals.ThePIXImusallowsautomatedaccurateandprecisemeasurementofboneandtissueforsmallanimals10-50g(e.g.micelemmings).Thesebonefatandleanmeasurementsexhibitexcellentcorrelationtototaltheirashedorchemicalextractionweights(r=0.990.930.99respectively).PIXImususesalowerx-rayenergythanthatusedforperipheraldensitometryinhumansinordertoachievecontrastintheextremelylowdensitybone.ExcellentprecisionofBMDand%FatmakesPIXImusidealforlongitudinalstudies.Withanimageareaof80mmx65mmthePIXImuscanimagetheentirebodyofmostmiceandthesubcranialregionoflargeobobmice.Thestandardtotalbodyresultfocusesonthesub-cranialregionimprovingsensitivitybyexcludingtheinactiveboneandtissueofthecranium.Therearemanualregionsofinterest(ROIs)forselectedareaswithinthetotalbodyimagesuchasspineandfemur.ResearchersusingtheratcanimagelargeportionsoftheanimalandpositionROIsovertheirareaofinterest(i.e.entirelegspineabdomen)producingbothboneandtissueresults.PIXImusisdrivenbysingle-keycommandsandagraphicaluserinterface.Asinglefunctionkeyperformsanautomatedimageacquisitionandanalysis.Subjectresultsareprintedinacleareasy-to-understandformatanddataarestoredelectronicallyinagloballyacceptedformat.SaveAnimals-A10-week&longitudinal&studyofbodycomposition-withweeklydatapointsandasamplesizeof10-require100animals(10sacrificedperweekfor10weeks).WhilethePIXImusonlyneeds10animalstobeimagedweeklyintruelongitudinalfashionsaving90animals.SaveTime-Short-termtimesavingswithPIXImusareobvious-thePIXImusneedsonly5minutestoacquireandanalyzeboneandtissueresultswhilechemicalextractionrequires~1man-hour(homogenizingscrapingcleaningweighingdryingchemicalextractionmoreweighing.).Longer-termsavingsarejustasdramaticwithchemicalextraction&sdurationrequiringdaysofdryingandashingtimemeanwhilethePIXImusiscompletein5minutes.YeswithPIXImusyoucanhaveyourdataTODAY!SaveMoney-ThePIXImussavesyourpreciousgrantmoneyswithlessanimalsandlaborrequirementsasdescribedabove.Butlookdeeper.Feweranimalsrequirelesscagesspaceanimalhandlinglaborfoodchemicalslabequipmentsavingsubstantialresearchdollarsandstreamliningyouroperation.ImproveConfidence-SufficienttimeandanimalsaresavedwiththePIXImusthatitisbothtime-andcost-efficienttodoubleoreventriplethesamplesizeofmostanystudy.Largersamplesizesmeanmorerobuststatisticsthatpleasereviewerstogetdatapublishedand/orproductstomarketfaster.#